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p153 pPr-cre
(Plasmid #8403)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8403 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pbeta-gal-Basic
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    probasin driven Cre
  • Insert Size (bp)
    1500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

428 bp probasin promoter fragment from prPb-tva950 (Pam Schwartzenberg, NCI), Cre from pBS185, coding sequence for beta-gal removed

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p153 pPr-cre was a gift from Jeffrey Green (Addgene plasmid # 8403 ; http://n2t.net/addgene:8403 ; RRID:Addgene_8403)
  • For your References section:

    A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk SJ, Green JE. Nucleic Acids Res 2001 Jun 15;29(12):E56-6. 10.1093/nar/29.12.e56 PubMed 11410679