Purpose(Empty Backbone) Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84586||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7500
Modifications to backboneTwo double FRT sites were inserted surrounding the multiple cloning site.
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCasper_F
- 3′ sequencing primer pCasper_R (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FoRC.PGm was a gift from Francesca Pignoni (Addgene plasmid # 84586 ; http://n2t.net/addgene:84586 ; RRID:Addgene_84586)
For your References section:Mutant Analysis by Rescue Gene Excision: New tools for mosaic studies in Drosophila. Zhou Q, Neal SJ, Pignoni F. Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984. 10.1002/dvg.22984 PubMed 27696669