pBsaBM3
(Plasmid
#85102)
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PurposeP450 BM3 variant (A74G/C773S/C810S)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHSG398
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Backbone manufacturerTaKaRa
- Backbone size w/o insert (bp) 2227
- Total vector size (bp) 5405
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Modifications to backboneN/A
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP450 BM3
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Alt nameCYP102A1
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SpeciesBacillus megaterium
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Insert Size (bp)3178
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MutationA74G/C773S/C810S
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GenBank IDWP_034650526.1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 (-40) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
P450 BM3 was originally cloned from Bacillus megaterium (NBRC 15308) in our lab
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBsaBM3 was a gift from Teruyuki Nagamune (Addgene plasmid # 85102 ; http://n2t.net/addgene:85102 ; RRID:Addgene_85102) -
For your References section:
Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support. Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T. Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033. 10.1002/anie.201608033 PubMed 27781345
