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Dat - EGFP - Tol2
(Plasmid #85418)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85418 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Electroporation is recommended for transformation due to plasmid size.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dopamine transporter
  • Alt name
    dat
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    27000
  • Entrez Gene
    slc6a3 (a.k.a. dat)
  • Promoter dat
  • Tag / Fusion Protein
    • EGFP

Cloning Information

Resource Information

Depositor Comments

Contains the dat genomic sequences, including approximately 13 kb of its 50-flanking region, but excluding the last coding exon and 30-flanking region. The EGFP coding sequence is cloned in frame into dat exon 1.

Due to large size, transformation and DNA prep efficiencies may be low. Electroporation is recommended for transformation and low copy prep protocols using large starting volumes are recommended for purifying the DNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dat - EGFP - Tol2 was a gift from Marc Ekker (Addgene plasmid # 85418 ; http://n2t.net/addgene:85418 ; RRID:Addgene_85418)
  • For your References section:

    Transgenic zebrafish expressing green fluorescent protein in dopaminergic neurons of the ventral diencephalon. Xi Y, Yu M, Godoy R, Hatch G, Poitras L, Ekker M. Dev Dyn. 2011 Nov;240(11):2539-47. doi: 10.1002/dvdy.22742. Epub 2011 Sep 19. 10.1002/dvdy.22742 PubMed 21932324