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pSkipFlox
(Plasmid #85797)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85797 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM
  • Vector type
    Cre/Lox ; P. falciparum Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    split Cre recombinase
  • Species
    Synthetic
  • Promoter Pfcrt
  • Tag / Fusion Protein
    • FKBP, FRB*

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer crtfw
  • 3′ sequencing primer pARLrv
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSkipFlox was a gift from Tobias Spielmann (Addgene plasmid # 85797 ; http://n2t.net/addgene:85797 ; RRID:Addgene_85797)
  • For your References section:

    A genetic system to study Plasmodium falciparum protein function. Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223. 10.1038/nmeth.4223 PubMed 28288121