Addgene - pcDNA3-FLAG-LKB1 Plasmid Data

Plasmid Cart

Login to add to cart

Recently Visited

8590: pcDNA3-FLAG-…
8513: HA Raptor
8455: pCMV-dR8.2 d…
8454: pCMV-VSV-G
8453: pLKO.1 puro

Popular Plasmids

Special Collections

Analyze Sequence

Vector Database

Plasmid 8590: pcDNA3-FLAG-LKB1

Gene/insert name:
LKB1
Insert size:
1400
Species:
H. sapiens (human)
GenBank ID:
Q15831
Entrez Gene:
STK11 (PJS, LKB1)
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert:
5400
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Lewis Cantley
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. Shaw et al (Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8590" in your Materials and Methods section.