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pNIC28-DENV3-NS5FL
(Plasmid #86013)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86013 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 7284
  • Total vector size (bp) 9960
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DENV3-NS5FL
  • Species
    DENV3
  • Insert Size (bp)
    2676
  • Mutation
    amino acid residues 6 to 895 of DENV3 NS5
  • GenBank ID
    AY662691.1
  • Promoter T7
  • Tag / Fusion Protein
    • hexa-histidine tag (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The NS5 insert in this plasmid contains minor discrepancies compared to DENV3 reference sequences in NCBI. The depositing lab has confirmed that this plasmid is functional for protein purification.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC28-DENV3-NS5FL was a gift from Dahai Luo (Addgene plasmid # 86013 ; http://n2t.net/addgene:86013 ; RRID:Addgene_86013)
  • For your References section:

    A crystal structure of the Dengue virus NS5 protein reveals a novel inter-domain interface essential for protein flexibility and virus replication. Zhao Y, Soh TS, Zheng J, Chan KW, Phoo WW, Lee CC, Tay MY, Swaminathan K, Cornvik TC, Lim SP, Shi PY, Lescar J, Vasudevan SG, Luo D. PLoS Pathog. 2015 Mar 16;11(3):e1004682. doi: 10.1371/journal.ppat.1004682. eCollection 2015 Mar. PPATHOGENS-D-14-02291 [pii] PubMed 25775415