pNIC28-DENV3-NS5FL
(Plasmid #86013)

• Purpose: Expression of dengue virus 3 NS5 protein
• Depositing Lab: Dahai Luo
• Publication: Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682. doi: 10.1371/journal.ppat.1004682. eCollection 2015 Mar.

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 7284
• Total vector size (bp): 9960
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DENV3-NS5FL
• Species: DENV3
• Insert Size (bp): 2676
• Mutation: amino acid residues 6 to 895 of DENV3 NS5
• GenBank ID: AY662691.1
• Promoter: T7
• Tag / Fusion Protein:
  • hexa-histidine tag (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The NS5 insert in this plasmid contains minor discrepancies compared to DENV3 reference sequences in NCBI. The depositing lab has confirmed that this plasmid is functional for protein purification.