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pSTAP-seq_fly-zfh1
(Plasmid #86381)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86381 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3426
  • Vector type
    STAP-seq screening vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D. melanogaster zfh1 enhancer
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    862

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTAP-seq_fly-zfh1 was a gift from Alexander Stark (Addgene plasmid # 86381 ; http://n2t.net/addgene:86381 ; RRID:Addgene_86381)
  • For your References section:

    Genome-wide assessment of sequence-intrinsic enhancer responsiveness at single-base-pair resolution. Arnold CD, Zabidi MA, Pagani M, Rath M, Schernhuber K, Kazmar T, Stark A. Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739. 10.1038/nbt.3739 PubMed 28024147