|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8661||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4874
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneUBQLN2 (a.k.a. ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p4455 FLAG-hPLIC-2 was a gift from Peter Howley (Addgene plasmid # 8661 ; http://n2t.net/addgene:8661 ; RRID:Addgene_8661)
For your References section:The hPLIC proteins may provide a link between the ubiquitination machinery and the proteasome. Kleijnen MF, Shih AH, Zhou P, Kumar S, Soccio RE, Kedersha NL, Gill G, Howley PM. Mol Cell 2000 Aug;6(2):409-19. 10.1016/S1097-2765(00)00040-X PubMed 10983987