4xM67 pTATA TK-Luc
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8688||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepTATA TK-Luc
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name4 repeated M67 sites
Insert Size (bp)98
- Cloning method Restriction Enzyme
- 5′ cloning site AccI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer lucNrev (Common Sequencing Primers)
To obtain p4xM67-tk-Luc, an oligonucleotide containing four copies of the sequence GGTTCCCGTAAATGCATCA (TTCCCGTAA is the STAT-binding site) was introduced in the AccI-BamHI sites of pTATA-tk-Luc upstream of the minimal promoter. Diagnostic digest is EcoRI (3.6kb, 1.7kb, 0.6kb).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:4xM67 pTATA TK-Luc was a gift from Jim Darnell (Addgene plasmid # 8688 ; http://n2t.net/addgene:8688 ; RRID:Addgene_8688)
For your References section:A single amino acid substitution in the v-Eyk intracellular domain results in activation of Stat3 and enhances cellular transformation. Besser D, Bromberg JF, Darnell JE Jr, Hanafusa H. Mol Cell Biol 1999 Feb;19(2):1401-9. PubMed 9891073