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Plasmid 8700: Stat1 alpha S727A pRc/CMV
  • STAT1 alpha S727A

  • STAT1a

  • STAT1 alpha

  • 2400

  • H. sapiens (human)

  • M97935

  • STAT1 (CANDF7, ISGF-3, STAT91)

  • S727A

  • pRc/CMV
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 5500

  • NotI

  • No

  • ApaI

  • No

  • T7 List of Sequencing Primers

  • SP6

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • View sequences (1)
  • View map

  • Jim Darnell

  • UBMTA

Comments: 

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb). Author's Map shows the wild-type version of this plasmid (without the S727A mutation).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Wen et al (Cell 1995 Jul 28;82(2):241-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8700" in your Materials and Methods section.