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Stat1 alpha S727A pRc/CMV
(Plasmid #8700)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.


  • Gene/Insert name
    STAT1 alpha S727A
  • Alt name
  • Alt name
    STAT1 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Entrez Gene
    STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb). Author's Map shows the wild-type version of this plasmid (without the S727A mutation).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat1 alpha S727A pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8700)
  • For your References section:

    Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Wen Z, Zhong Z, Darnell JE Jr. Cell 1995 Jul 28;82(2):241-50. 10.1016/0092-8674(95)90311-9 PubMed 7543024