Addgene - Stat1 alpha S727A pRc/CMV Plasmid Data

Plasmid 8700: Stat1 alpha S727A pRc/CMV

Gene/insert name:
STAT1 alpha S727A
Alt name:
STAT1a
Alt name:
STAT1 alpha
Insert size:
2400
Species:
H. sapiens (human)
GenBank ID:
M97935
Entrez Gene:
STAT1 (CANDF7, ISGF-3, STAT91)
Mutation:
S727A
Vector backbone:
pRc/CMV
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
ApaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jim Darnell
Terms and Licenses:
MTA

Comments:

Diagnostic Restriction Digest with HindIII (6.1kb and 1.7kb). Author's Map shows the wild-type version of this plasmid (without the S727A mutation).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Wen et al (Cell 1995 Jul 28;82(2):241-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8700" in your Materials and Methods section.