Stat3 S727A pRc/CMV
(Plasmid #8708)

• Depositing Lab: Jim Darnell
• Publication: Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062-7.

Backbone

• Vector backbone: pRc/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Stat3 S727A
• Alt name: Stat3
• Species: M. musculus (mouse)
• Insert Size (bp): 3000
• Mutation: S727A (exhibits o serine phosphorylation either constitutive or induced) *see comments
• GenBank ID: U06922
• Entrez Gene: Stat3 (a.k.a. 1110034C02Rik, Aprf)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Diagnostic Restriction Digest with BamHI (3.2kb, 3.1kb, and 2.0kb).

* E16K mutation found during QC