pET28b-pchG WT
(Plasmid #87107)

• Purpose: expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa
• Depositing Lab: Audrey Lamb
• Publication: Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33. doi: 10.1021/acs.biochem.6b00735. Epub 2016 Sep 15.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5368
• Total vector size (bp): 6418
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Growth instructions: 20 hours growth after induction
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pchG WT
• Species: Pseudomonas aeruginosa PAO1
• Insert Size (bp): 1050
• Promoter: T7
• Tag / Fusion Protein:
  • his-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pET upstream
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry