pcDNA3.1_nHA Spinophilin
(Plasmid
#87122)
-
Purposemammalian expression of Spinophilin
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepcDNA3.1/nHA-DEST
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSpinophilin
-
SpeciesH. sapiens (human)
-
Entrez GenePPP1R9B (a.k.a. PPP1R9)
- Promoter CMV
-
Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1_nHA Spinophilin was a gift from Anthony Baucum (Addgene plasmid # 87122 ; http://n2t.net/addgene:87122 ; RRID:Addgene_87122) -
For your References section:
Mechanisms and Consequences of Dopamine Depletion-Induced Attenuation of the Spinophilin/Neurofilament Medium Interaction. Hiday AC, Edler MC, Salek AB, Morris CW, Thang M, Rentz TJ, Rose KL, Jones LM, Baucum AJ 2nd. Neural Plast. 2017;2017:4153076. doi: 10.1155/2017/4153076. Epub 2017 May 28. 10.1155/2017/4153076 PubMed 28634551
