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pGADT7 Hsh155 K335N
(Plasmid #87298)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87298 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGADT7
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7987
  • Total vector size (bp) 10910
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HSH155
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2923
  • Mutation
    K335N
  • GenBank ID
    NP_014015.1
  • Entrez Gene
    HSH155 (a.k.a. YMR288W)
  • Tags / Fusion Proteins
    • GAL4 AD (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CCAGTGAATTCCACCCGGGAATGAGTCATCCGATTCAATTTG
  • 3′ sequencing primer GCTCGAGCTCGATGGATCCTCACAGAACTAAATCCAGTTCTTC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGADT7 Hsh155 K335N was a gift from Aaron Hoskins (Addgene plasmid # 87298 ; http://n2t.net/addgene:87298 ; RRID:Addgene_87298)
  • For your References section:

    SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA. Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349. 10.1093/nar/gkw1349 PubMed 28062854