|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8839||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5700
Modifications to backboneDNA encoding presumptive N-terminal signal peptide deleted.
Vector typeBacterial Expression
/ Fusion Protein
- T. litoralis MBP (N terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsBecause it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
Copy numberLow Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer pBRrevBam; T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of T. litoralis maltose-binding protein. Presumptive N-terminal signal peptide deleted.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKM999 was a gift from David Waugh (Addgene plasmid # 8839)
For your References section:Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox JD, Routzahn KM, Bucher MH, Waugh DS. FEBS Lett. 2003 Feb 27. 537(1-3):53-7. 10.1016/S0014-5793(03)00070-X PubMed 12606030