|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89178||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepYUB28b Addgene #37277
Backbone manufacturerGhader Bashiri
- Backbone size (bp) 6040
Vector typeBacterial Expression ; mycobacteria shuttle vector
/ Fusion Protein
- His-MBP-TEV (N terminal on backbone)
Growth in Bacteria
- Promoter T7
- Cloning method Unknown
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
Note that the MBP tag in this plasmid is a surface entropy reduced version of the tag in order to help crytallisation down the line, and therefore has 6 alanine substitutions compared to the standard MBP sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYUB28b-MBP was a gift from Ted Baker & Ghader Bashiri (Addgene plasmid # 89178 ; http://n2t.net/addgene:89178 ; RRID:Addgene_89178)
For your References section:Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. 10.1371/journal.pone.0015803 PubMed 21209917