pYUB28b-MBP
(Plasmid #89178)

• Purpose: (Empty Backbone) mycobacteria shuttle vector
• Depositing Labs: Ted Baker, Ghader Bashiri
• Publication: Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Backbone

• Vector backbone: pYUB28b Addgene #37277
• Backbone manufacturer: Ghader Bashiri
• Backbone size (bp): 6040
• Vector type: Bacterial Expression ; mycobacteria shuttle vector
• Tag / Fusion Protein:
  • His-MBP-TEV (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: none
• Promoter: T7

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.

Note that the MBP tag in this plasmid is a surface entropy reduced version of the tag in order to help crytallisation down the line, and therefore has 6 alanine substitutions compared to the standard MBP sequence.