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pYUB28b-MBP
(Plasmid #89178)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89178 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYUB28b Addgene #37277
  • Backbone manufacturer
    Ghader Bashiri
  • Backbone size (bp) 6040
  • Vector type
    Bacterial Expression ; mycobacteria shuttle vector
  • Tag / Fusion Protein
    • His-MBP-TEV (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    none
  • Promoter T7

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.

Note that the MBP tag in this plasmid is a surface entropy reduced version of the tag in order to help crytallisation down the line, and therefore has 6 alanine substitutions compared to the standard MBP sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYUB28b-MBP was a gift from Ted Baker & Ghader Bashiri (Addgene plasmid # 89178 ; http://n2t.net/addgene:89178 ; RRID:Addgene_89178)
  • For your References section:

    Metabolic engineering of cofactor F420 production in Mycobacterium smegmatis. Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN. PLoS One. 2010 Dec 29;5(12):e15803. 10.1371/journal.pone.0015803 PubMed 21209917