|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89180||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBroad Insitute
- Backbone size (bp) 7943
Modifications to backbonepCW57-MCS1-2A-MCS2 was modified by replacing puromycin-T2A-rtTA with rtTA-T2A-neomycin.
Vector typeMammalian Expression, Lentiviral ; Doxycycline inducible; P2A Self Cleaving Peptide
- Promoter TRE
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth Strain(s)NEB Stable
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCW57.MCS_Seq_F CGTATGTCGAGGTAGGCGTG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCW57-MCS1-P2A-MCS2 (Neo) was a gift from Adam Karpf (Addgene plasmid # 89180 ; http://n2t.net/addgene:89180 ; RRID:Addgene_89180)