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pCW57-MCS1-P2A-MCS2 (Neo)
(Plasmid #89180)


Item Catalog # Description Quantity Price (USD)
Plasmid 89180 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Broad Insitute
  • Backbone size (bp) 7943
  • Modifications to backbone
    pCW57-MCS1-2A-MCS2 was modified by replacing puromycin-T2A-rtTA with rtTA-T2A-neomycin.
  • Vector type
    Mammalian Expression, Lentiviral ; Doxycycline inducible; P2A Self Cleaving Peptide
  • Promoter TRE
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57-MCS1-P2A-MCS2 (Neo) was a gift from Adam Karpf (Addgene plasmid # 89180 ; ; RRID:Addgene_89180)