PurposeExpresses GPA1 under control of GAL1 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89201||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbone604 TRP
Vector typeyeast integration
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Entrez GeneGPA1 (a.k.a. YHR005C, CDC70, DAC1, SCG1)
- Promoter GAl1
- Cloning method Restriction Enzyme
- 5′ cloning site PSPOMI (unknown if destroyed)
- 3′ cloning site BAMHI (unknown if destroyed)
- 5′ sequencing primer nonstandard (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
linearize by digesting with Pme1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHES939 was a gift from Hana El-Samad (Addgene plasmid # 89201 ; http://n2t.net/addgene:89201 ; RRID:Addgene_89201)
For your References section:Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast. Aranda-Diaz A, Mace K, Zuleta I, Harrigan P, El-Samad H. ACS Synth Biol. 2016 Dec 27. doi: 10.1021/acssynbio.6b00251. 10.1021/acssynbio.6b00251 PubMed 27930885