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pG5ElbLuc
(Plasmid #89341)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89341 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pG5ElbLuc
  • Backbone size (bp) 4955
  • Modifications to backbone
    The plasmid pG5ElbLuc was prepared from pG5ElbCAT (Lillie and Green, 1989) by removing the CAT gene by restriction digestion (EcoRI and Styl) and inserting the firefly luciferase gene (from pGEM-Luc) as a blunt-ended 1745-bp StuI-Hind111 fragment.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG5ElbLuc was a gift from Roger Davis (Addgene plasmid # 89341 ; http://n2t.net/addgene:89341 ; RRID:Addgene_89341)
  • For your References section:

    Signal transduction within the nucleus by mitogen-activated protein kinase. Seth A, Gonzalez FA, Gupta S, Raden DL, Davis RJ. J Biol Chem. 1992 Dec 5;267(34):24796-804. PubMed 1332967