CRY2-GFP-homer1c
(Plasmid #89442)

• Purpose: Localizes CRY2 to excitatory synapses
• Depositing Lab: Matthew Kennedy
• Publication: Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26.
• Sequence Information:

  • Depositor Sequences: Full (1)
  • Addgene Sequences: Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	89442	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pCAG
  (Search Vector Database)
• Backbone manufacturer: custom
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2-GFP-homer1c
• Alt name: zip45
• Species: R. norvegicus (rat), A. thaliana (mustard weed)
• Mutation: Contains Full length CRY2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • CRY2_EGFP_Homer1c_pCAG_MKp415.gb
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials

Depositor Comments

strong promoter-high expression levels lead to aggregation. Use~150ng plasmid for one well of a 12 well plate