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PurposeLocalizes CRY2 to excitatory synapses
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 89442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAG
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Backbone manufacturercustom
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRY2-GFP-homer1c
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Alt namezip45
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SpeciesR. norvegicus (rat), A. thaliana (mustard weed)
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MutationContains Full length CRY2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Depositor Comments
strong promoter-high expression levels lead to aggregation. Use~150ng plasmid for one well of a 12 well plate
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CRY2-GFP-homer1c was a gift from Matthew Kennedy (Addgene plasmid # 89442 ; http://n2t.net/addgene:89442 ; RRID:Addgene_89442) -
For your References section:
Optogenetic Control of Synaptic Composition and Function. Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, Dell'Acqua ML, Kennedy MJ. Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26. 10.1016/j.neuron.2016.12.037 PubMed 28132827