|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89477||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVictor de Lorenzo lab
Modifications to backboneTn5 replaced with marC9. Mariner transposon ends cloned into the transposon cassette. The transposon ends are mutagenized to introduce an mmeI cut site.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Growth instructionsUse BW29427 strain supplemented with 300uM DAP for this plasmid when conjugating from E. coli to Vibrio natriegens
BW29427 is an E. coli strain with diaminopimelic acid (DAP) auxotrophy. BW29427 growth requires 300uM DAP even when cultured in LB. Importantly, BW29427 does not grow in the absence of DAP, which simplifies counterselection of this host strain following biparental mating with Vibrio natriegens.
Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMarC9-R6k was a gift from George Church (Addgene plasmid # 89477 ; http://n2t.net/addgene:89477 ; RRID:Addgene_89477)
For your References section:Vibrio natriegens, a new genomic powerhouse. Lee HH, Ostrov N, Wong BG, Gold MA, Khalil A, Church GM. bioRxiv 058487 10.1101/058487