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YFC43
(Plasmid #89488)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89488 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMDC43
  • Vector type
    Plant Expression
  • Promoter 2x35S
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • YFP-C (aa 154-240) (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    These pMDC43 derived plasmids have genes of resistance not only to Kanamycin but also to Amikacin, while the genes of resistance to Kan in the pDONR don't have it. So this way the recombinant clones between pYFN43 or pYFC43 with pDONr can be differentiated.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Depositor Comments

To enhance folding of the fusion proteins a flexible linker was introduced between the coding sequence of either fragment of YFP and the attR recombination site. For more information see: http://www.ibmcp.upv.es/FerrandoLabVectors/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFC43 was a gift from Alejandro Ferrando (Addgene plasmid # 89488 ; http://n2t.net/addgene:89488 ; RRID:Addgene_89488)
  • For your References section:

    Aminopropyltransferases involved in polyamine biosynthesis localize preferentially in the nucleus of plant cells. Belda-Palazon B, Ruiz L, Marti E, Tarraga S, Tiburcio AF, Culianez F, Farras R, Carrasco P, Ferrando A. PLoS One. 2012;7(10):e46907. doi: 10.1371/journal.pone.0046907. Epub 2012 Oct 8. 10.1371/journal.pone.0046907 PubMed 23056524