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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
(Plasmid #89508)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89508 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5379
  • Total vector size (bp) 10528
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, transform into Rosetta (DE3) Competent cells. Induce at 0.6 OD with 1 mM IPTG final concentration. Growing overnight (12~14 hours) at 18 C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1-nCas9 (D10A)-UGI-NLS
  • Alt name
    rAPOBEC1-nCas9-UGI-NLS
  • Species
    R. norvegicus (rat); Streptococcus pyogenes (bacteria)
  • Insert Size (bp)
    5149
  • Mutation
    D10A mutation of Cas9
  • Promoter T7
  • Tags / Fusion Proteins
    • rAPOBEC-XTEN (N terminal on insert)
    • UGI (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS was a gift from Jin-Soo Kim (Addgene plasmid # 89508 ; http://n2t.net/addgene:89508 ; RRID:Addgene_89508)
  • For your References section:

    Highly efficient RNA-guided base editing in mouse embryos. Kim K, Ryu SM, Kim ST, Baek G, Kim D, Lim K, Chung E, Kim S, Kim JS. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816. 10.1038/nbt.3816 PubMed 28244995