Addgene - pcDNA3-FLAG-Fos Y345A Plasmid Data

Plasmid 8970: pcDNA3-FLAG-Fos Y345A

Gene/insert name:
c-Fos
Alt name:
Fos
Insert size:
1200
Species:
R. norvegicus (rat)
Entrez Gene:
Fos (c-fos)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
Y345A
Vector backbone:
pcDNA3.1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
John Blenis
Terms and Licenses:
MTA

Comments:

Mutation in c-Fos DEF domain (FTYP).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Molecular interpretation of ERK signal duration by immediate early gene products. Murphy et al (Nat Cell Biol. 2002 Aug . 4(8):556-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8970" in your Materials and Methods section.