Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

pcDNA3-FLAG-Fos Y345A
(Plasmid #8970)

Loading...
Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    c-Fos
  • Alt name
    Fos
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Mutation
    Y345A
  • Entrez Gene
    Fos (a.k.a. c-fos)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Mutation in c-Fos DEF domain (FTYP).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-Fos Y345A was a gift from John Blenis (Addgene plasmid # 8970)
  • For your References section:

    Molecular interpretation of ERK signal duration by immediate early gene products. Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J. Nat Cell Biol. 2002 Aug . 4(8):556-64. 10.1038/ncb822 PubMed 12134156