p16L1-GFP
(Plasmid #89910)

• Purpose: Self-packages in TT-16L2 stable line. L1-only capsid propagation.
• Depositing Lab: John Schiller
• Publication: Buck et al J Virol. 2005 Mar;79(5):2839-46.

Backbone

• Vector backbone: many different ones
• Backbone manufacturer: Invitrogen
• Vector type: Unspecified
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HPV16L1+ GFP
• Alt name: phüLsf
• Species: H. sapiens (human)
• Promoter: SV40 and hEF1alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (unknown if destroyed)
• 3′ cloning site: Pst1 (unknown if destroyed)
• 5′ sequencing primer: AGGCTCATGG

Resource Information

• Supplemental Documents:
  • P16L1-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry