Purpose(Empty Backbone) Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89916||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 1966
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Unknown
- 5′ sequencing primer GTAAAACGACGGCCAGTT
- 3′ sequencing primer CAGGAAACAGCTATGAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BB1_L_34_syn_BsaI was a gift from Michael Sauer (Addgene plasmid # 89916 ; http://n2t.net/addgene:89916 ; RRID:Addgene_89916)
For your References section:An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. 10.1016/j.biortech.2017.05.004 PubMed 28533066