Addgene - 1051 pBabe puroL Akt Plasmid Data

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Plasmid 9011: 1051 pBabe puroL Akt

Gene/insert name:
Akt1
Alt name:
Akt
Insert size:
1500
Species:
H. sapiens (human)
Entrez Gene:
AKT1 (AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pBabe puro L
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
5200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBabe5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. DiRenzo et al (Cancer Res. 2002 Jan 1. 62(1):89-98. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9011" in your Materials and Methods section.