pSL14 - Prp8 2142–2398
(Plasmid
#90264)
-
Purposeprotein expression
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90264 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepDEST15
-
Backbone manufacturerThermoFisher Scientific
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePRP8
-
Alt nameRNA8
-
Alt nameSLT21
-
SpeciesS. cerevisiae (budding yeast)
-
Mutationonly Jab1 domain (aa 2143-2398)
-
Entrez GenePRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)
- Promoter T7
-
Tag
/ Fusion Protein
- N-terminal 6xHis tag (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 forward (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ledoux and Guthrie, JBC, 2016
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSL14 - Prp8 2142–2398 was a gift from Christine Guthrie (Addgene plasmid # 90264 ; http://n2t.net/addgene:90264 ; RRID:Addgene_90264) -
For your References section:
Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. Ledoux S, Guthrie C. J Biol Chem. 2016 Jun 3;291(23):11954-65. doi: 10.1074/jbc.M115.710848. Epub 2016 Apr 12. 10.1074/jbc.M115.710848 PubMed 27072132
