Purpose(Empty Backbone) BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSI
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90275||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3147
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Unknown
- 5′ sequencing primer CATTAATGCAGCTGGCAC
- 3′ sequencing primer GGTTATTGTCTCATGAGCGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BB2_L_12_syn_A_I was a gift from Michael Sauer (Addgene plasmid # 90275 ; http://n2t.net/addgene:90275 ; RRID:Addgene_90275)
For your References section:An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. 10.1016/j.biortech.2017.05.004 PubMed 28533066