Addgene - 1201 pAdTrack FKHR H215R AAA Plasmid Data

Plasmid 9039: 1201 pAdTrack FKHR H215R AAA

Gene/insert name:
FKHR H215R AAA
Alt name:
FKHR
Alt name:
FOXO1A
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
FOXO1 (FKH1, FKHR, FOXO1A)
Fusion protein or tag:
flag
Terminal:
N terminal on insert
Mutation:
H215R T24A S256A S319A
Vector backbone:
pAdTrack CMV
(Search Vector Database)
Backbone manufacturer:
ATCC
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert (bp):
9010
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
pcDNA flag FKHR was a gift from Eric Tang.
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Comments:

Inserts liberated by partial HindIII and complete XbaI digestion of pCDNA3-Flag-FKHR were ligated to HindIII/XbaI-restricted pAD-TrackCMV to give pAD-Track-Flag-FKHR. Must recombine into pAdEasy before generating Adenoviral particles.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy et al (Cancer Cell. 2002 Jul . 2(1):81-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9039" in your Materials and Methods section.