1201 pAdTrack FKHR H215R AAA
(Plasmid #9039)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAdTrack CMV
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 9010
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    FKHR H215R AAA
  • Alt name
    FKHR
  • Alt name
    FOXO1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    H215R T24A S256A S319A
  • Entrez Gene
    FOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA flag FKHR was a gift from Eric Tang.
  • Terms and Licenses

Depositor Comments

Inserts liberated by partial HindIII and complete XbaI digestion of pCDNA3-Flag-FKHR were ligated to HindIII/XbaI-restricted pAD-TrackCMV to give pAD-Track-Flag-FKHR. Must recombine into pAdEasy before generating Adenoviral particles.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1201 pAdTrack FKHR H215R AAA was a gift from William Sellers (Addgene plasmid # 9039)
  • For your References section:

    A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy S, Nakamura N, Sansal I, Bergeron L, Sellers WR. Cancer Cell. 2002 Jul . 2(1):81-91. 10.1016/S1535-6108(02)00086-7 PubMed 12150827