1
Plasmid 9039: 1201 pAdTrack FKHR H215R AAA
  • FKHR H215R AAA

  • FKHR

  • FOXO1A

  • 2000

  • H. sapiens (human)

  • FOXO1 (FKH1, FKHR, FOXO1A)

  • flag

  • N terminal on insert

  • H215R T24A S256A S319A

  • pAdTrack CMV
    (Search Vector Database)

  • ATCC

  • Mammalian Expression, Adenoviral

  • 9010

  • HindIII

  • No

  • XbaI

  • No

  • n/a List of Sequencing Primers

  • Kanamycin

  • DH5alpha

  • 37

  • High Copy

  • Neomycin

  • pcDNA flag FKHR was a gift from Eric Tang.

  • View sequences (3)
  • View map

  • William Sellers

  • UBMTA

Comments: 

Inserts liberated by partial HindIII and complete XbaI digestion of pCDNA3-Flag-FKHR were ligated to HindIII/XbaI-restricted pAD-TrackCMV to give pAD-Track-Flag-FKHR. Must recombine into pAdEasy before generating Adenoviral particles.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy et al (Cancer Cell. 2002 Jul . 2(1):81-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9039" in your Materials and Methods section.