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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 9044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | ||
Cloning Grade DNA | 9044-DNA.cg | 2 µg of cloning grade DNA in Tris buffer | 1 | $95 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonemodified pMSCV 2.2
- Backbone size (bp) 6500
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Alt nameMSCV IRES GFP
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Tag
/ Fusion Protein
- IRES GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
Excised IRES hGFP-3M from pCITE IRES hGFP-3M with EcoRI/SalI digest. Ligated into MSCV2.2 following EcorI/SalI digest of vector. Lost puro resistance gene (pac) in process. Luk Van Parijs et al., Immunity 11(3): 281.
Also see Addgene Plasmid #12282, pMIG-w, which is a variant of pMIG containing a WRE element. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.
Please see plasmid 20672: MSCV-IRES-GFP for sequence reference.
Information for Cloning Grade DNA (Catalog # 9044-DNA.cg) ( Back to top )
Purpose
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
Delivery
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
Quality Control
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIG was a gift from William Hahn (Addgene plasmid # 9044 ; http://n2t.net/addgene:9044 ; RRID:Addgene_9044)