pMIG
(Plasmid #9044)

• Purpose: (Empty Backbone)
• Depositing Lab: William Hahn
• Publication: Viral vectors. (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	9044	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart
Cloning Grade DNA	9044-DNA.cg	2 µg of cloning grade DNA in Tris buffer More Information	1	$95	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: modified pMSCV 2.2
  (Search Vector Database)
• Backbone size (bp): 6500
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Alt name: MSCV IRES GFP
• Tag / Fusion Protein:
  • IRES GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'

Resource Information

• Supplemental Documents:
  • pMIG-SalIEcoRI
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 37 References

Depositor Comments

Excised IRES hGFP-3M from pCITE IRES hGFP-3M with EcoRI/SalI digest. Ligated into MSCV2.2 following EcorI/SalI digest of vector. Lost puro resistance gene (pac) in process. Luk Van Parijs et al., Immunity 11(3): 281.

Also see Addgene Plasmid #12282, pMIG-w, which is a variant of pMIG containing a WRE element. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.

Please see plasmid 20672: MSCV-IRES-GFP for sequence reference.

Information for Cloning Grade DNA (Catalog # 9044-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $95 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here