pLO86
(Plasmid
#91724)
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Purpose(Empty Backbone) mycobacterial expression of mCherry reporter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneunknown
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Vector typeUnspecified ; mycobacterial expression
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLO86 was a gift from Graham Hatfull (Addgene plasmid # 91724 ; http://n2t.net/addgene:91724 ; RRID:Addgene_91724) -
For your References section:
Mutational analysis of the mycobacteriophage BPs promoter PR reveals context-dependent sequences for mycobacterial gene expression. Oldfield LM, Hatfull GF. J Bacteriol. 2014 Oct;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. 10.1128/JB.01801-14 PubMed 25092027
