CPH3488 (YCp LEU2 Rpb1 P1494A)
(Plasmid #91810)

• Purpose: Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation
• Depositing Lab: Chris Hill
• Publication: Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.

Backbone

• Vector backbone: pRS315
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 7771
• Total vector size (bp): 12970
• Modifications to backbone: Addition of endogenous RPB1 promoter and 3’ untranslated regions
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RPO21
• Alt name: RPB1, RPB220, SUA8, B220
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 5199
• Mutation: changed proline 1494 to alanine
• GenBank ID: NM_001180200.1
• Entrez Gene: RPO21 (a.k.a. YDL140C, RPB1, RPB220, SIT1, SUA8)
• Promoter: Endogenous Rpb1 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sph1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: CTGTTTCCAACACCCAT
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • CPH3488 (YCp LEU2 Rpb1 P1494A).gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was derived from pCK859, a gift from Craig Kaplan at Texas A&M University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry