CPH3495 (pET151_Spt6 1247-1451 K1435A)
(Plasmid #91817)

• Purpose: Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation
• Depositing Lab: Chris Hill
• Publication: Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 10.7554/eLife.28723.

Backbone

• Vector backbone: pET151/D-TOPO
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 5766
• Total vector size (bp): 6381
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SPT6
• Alt name: CRE2
• Alt name: SSN20
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 615
• Mutation: changed lysine 1435 to alanine; deleted residues 1-1246
• GenBank ID: NM_001181245.1
• Entrez Gene: SPT6 (a.k.a. YGR116W, CRE2, SSN20)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7trm

Resource Information

• Supplemental Documents:
  • CPH3495 (pET151_Spt6 1247-1451 K1435A).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry