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pSFV3
(Plasmid #92072)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92072 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP6-SFV4
  • Modifications to backbone
    Deletion AsuII to NdeI which removes the structural viral proteins.
  • Vector type
    Empty expression plasmid, for run-off transcription and RNA transfection of mammalian cells

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Deposited for prof. Henrik Garoff.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSFV3 was a gift from Henrik Garoff (Addgene plasmid # 92072 ; http://n2t.net/addgene:92072 ; RRID:Addgene_92072)
  • For your References section:

    A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Liljestrom P, Garoff H. Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed 1370252