pRS315-PGK-SEIPIN1
(Plasmid #96986)

• Purpose: Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.
• Depositing Lab: Kent Chapman
• Publication: Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 2015 Sep 11.

Backbone

• Vector backbone: pRS315-PGK
• Backbone size w/o insert (bp): 7840
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SEIPIN1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1104
• Entrez Gene: AT5G16460 (a.k.a. AT5G16460, MQK4.19, MQK4_19)
• Promoter: Phosphoglycerate Kinase (PGK) promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: PstI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutations V69A and D300N in LEU2 were found during Addgene's quality control process. The depositor noted that these mutations do NOT affect plasmid function.