AAV_Nanog HMEJ donor
PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||97321||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Mouse Targeting
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameNanog HMEJ donor
Alt nameNanog HMEJ template
Insert Size (bp)2437
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
gRNA sequence: cgtaagtctcatatttcacc
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV_Nanog HMEJ donor was a gift from Hui Yang (Addgene plasmid # 97321 ; http://n2t.net/addgene:97321 ; RRID:Addgene_97321)
For your References section:Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang P, Sun Q, Shi L, Yang H. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. 10.1038/cr.2017.76 PubMed 28524166