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pBa-LSS-GFP-LDLR wt
(Plasmid #98184)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98184 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBa-eGFP
  • Backbone size w/o insert (bp) 6755
  • Total vector size (bp) 9335
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LDLR
  • Alt name
    human low density lipoprotein receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3326
  • Mutation
    none
  • GenBank ID
    NM_000527
  • Entrez Gene
    LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
  • Promoter Chicken Beta Actin
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • LDLR signal sequence, N-term of GFP so GFP remains on mature protein when ss is cleaved (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII-vector, NheI-insert (destroyed during cloning)
  • 3′ cloning site RsrII (not destroyed)
  • 5′ sequencing primer TTCGGCTTCTGGCGTGTGAC
  • 3′ sequencing primer GATGAGTTTGGACAAACCAC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBa-LSS-GFP-LDLR wt was a gift from Gary Banker & Marvin Bentley (Addgene plasmid # 98184 ; http://n2t.net/addgene:98184 ; RRID:Addgene_98184)
  • For your References section:

    A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations. Jenkins B, Decker H, Bentley M, Luisi J, Banker G. J Cell Biol. 2012 Aug 20;198(4):749-61. doi: 10.1083/jcb.201205070. 10.1083/jcb.201205070 PubMed 22908316