|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||98514||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 1936
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAOX1 promoter
Insert Size (bp)928
MutationInternal BsaI and BpiI sites removed from insert
- Cloning method Restriction Enzyme
- 5′ cloning site BpiI (destroyed during cloning)
- 3′ cloning site BpiI (destroyed during cloning)
- 5′ sequencing primer 5'-GTAAAACGACGGCCAGTT-3' (M13-Fwd)
- 3′ sequencing primer 5´-CAGGAAACAGCTATGAC-3' (M13-Rev) (Common Sequencing Primers)
Please note that some discrepancies in the RPL2A promoter sequence were found during Addgene's Quality Control Process. The depositing lab noted that these discrepancies are likely due to errors in the initial genome sequence and should NOT affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BB1_12_pAOX1 was a gift from Brigitte Gasser & Diethard Mattanovich & Michael Sauer (Addgene plasmid # 98514 ; http://n2t.net/addgene:98514 ; RRID:Addgene_98514)
For your References section:GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris. Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, Sauer M, Mattanovich D, Gasser B, Marx H. BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3. 10.1186/s12918-017-0492-3 PubMed 29221460