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pLgw NKX2-5Y191C-V5-EcoDam
(Plasmid #98599)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98599 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLgw RFC1-V5-EcoDam
  • Backbone manufacturer
    PMID 9733859
  • Vector type
    Mammalian Expression, Lentiviral ; DamID
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NKX2-5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    957
  • Mutation
    Y191C point mutant
  • Entrez Gene
    Nkx2-5 (a.k.a. Csx, Nkx-2.5, Nkx2.5, tinman)
  • Promoter Heat Shock Minimal Promoter
  • Tag / Fusion Protein
    • V5 and Dam (DNA adenine methyltransferase) (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLgw NKX2-5Y191C-V5-EcoDam was a gift from Richard Harvey (Addgene plasmid # 98599 ; http://n2t.net/addgene:98599 ; RRID:Addgene_98599)
  • For your References section:

    NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets. Bouveret R, Waardenberg AJ, Schonrock N, Ramialison M, Doan T, de Jong D, Bondue A, Kaur G, Mohamed S, Fonoudi H, Chen CM, Wouters MA, Bhattacharya S, Plachta N, Dunwoodie SL, Chapman G, Blanpain C, Harvey RP. Elife. 2015 Jul 6;4. doi: 10.7554/eLife.06942. 10.7554/eLife.06942 PubMed 26146939