TVT7R(0,0)
(Plasmid
#98631)
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Purpose(Empty Backbone) Vector for cloning infectious RNA virus genomes
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAndy Ball’s Vector 2,0
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Transcription vector-T7R 0,0 has been designed to allow easy cloning of DNA to be transcribed from a T7 promoter to give an RNA with authentic 5’ and 3’ (by way of delta ribozyme cleavage) ends. The vector is based on Andy Ball's Vector 2,0 and uses remote recognition restriction enzymes to produce 4 bp 5’ overhangs at each end of the vector into which a DNA of interest can be inserted. For information on cloning sites please see Johnson etal Journal of Virology Vol 74 pp5123-5132
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TVT7R(0,0) was a gift from Andrew Ball & Karyn Johnson (Addgene plasmid # 98631 ; http://n2t.net/addgene:98631 ; RRID:Addgene_98631) -
For your References section:
Characterization and construction of functional cDNA clones of Pariacoto virus, the first Alphanodavirus isolated outside Australasia. Johnson KN, Zeddam JL, Ball LA. J Virol. 2000 Jun;74(11):5123-32. PubMed 10799587
Map uploaded by the depositor.
