pLV-shScn4b-268
(Plasmid
#99031)
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PurposeExpresses a shRNA targeting the mouse Scn4b gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLL3.7
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Backbone manufacturerMIT
- Backbone size w/o insert (bp) 7649
- Total vector size (bp) 7700
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Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScn4b shRNA
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gRNA/shRNA sequenceGACCCTAAGGTGAGAGTGA
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SpeciesM. musculus (mouse)
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GenBank IDNM_001013390.2
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Entrez GeneScn4b (a.k.a. Gm1471)
- Promoter mouse U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer U6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated as part of the NIAAA-funded INIA consortium.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-shScn4b-268 was a gift from Amy Lasek (Addgene plasmid # 99031 ; http://n2t.net/addgene:99031 ; RRID:Addgene_99031) -
For your References section:
The Sodium Channel beta4 Auxiliary Subunit Selectively Controls Long-Term Depression in Core Nucleus Accumbens Medium Spiny Neurons. Ji X, Saha S, Gao G, Lasek AW, Homanics GE, Guildford M, Tapper AR, Martin GE. Front Cell Neurosci. 2017 Feb 13;11:17. doi: 10.3389/fncel.2017.00017. eCollection 2017. 10.3389/fncel.2017.00017 PubMed 28243192
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