PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99550||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGietz & Sugino (1988) Gene 74:527-34
- Backbone size w/o insert (bp) 3545
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1569
Entrez GenePOL30 (a.k.a. YBR088C)
- Promoter POL30
/ Fusion Protein
- 6xHis (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SphI (unknown if destroyed)
- 5′ sequencing primer M13fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The ORF is followed by the ADH1 transcription terminator; digest with Bsu36I for integration into TRP1. Please see secondary publication PMID: 19107412 for more information about this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YIp204-P30-His-POL30 was a gift from Helle Ulrich (Addgene plasmid # 99550 ; http://n2t.net/addgene:99550 ; RRID:Addgene_99550)
For your References section:Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD. Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36. 10.1007/978-1-61779-998-3_36 PubMed 22941627