pLenti-TRE-dTomato-GCaMP6s
(Plasmid
#99864)
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Purposeexpresses dTomato and GCaMP6s under control of the Tet-off system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedTomato, GCaMP6s
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Insert Size (bp)1400
- Promoter Tet-responsive promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-TRE-dTomato-GCaMP6s was a gift from Venkatesh Murthy (Addgene plasmid # 99864 ; http://n2t.net/addgene:99864 ; RRID:Addgene_99864) -
For your References section:
Microglial depletion disrupts normal functional development of adult-born neurons in the olfactory bulb. Wallace J, Lord J, Dissing-Olesen L, Stevens B, Murthy VN. Elife. 2020 Mar 9;9. pii: 50531. doi: 10.7554/eLife.50531. 10.7554/eLife.50531 PubMed 32150529
