Adam Cohen Lab Plasmids


The Adam Cohen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam V, Cohen AE Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020.
Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. Venkatachalam V, Brinks D, Maclaurin D, Hochbaum D, Kralj J, Cohen AE J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27.
Screening fluorescent voltage indicators with spontaneously spiking HEK cells. Park J, Werley CA, Venkatachalam V, Kralj JM, Dib-Hajj SD, Waxman SG, Cohen AE PLoS One. 2013 Dec 31;8(12):e85221. doi: 10.1371/journal.pone.0085221. eCollection 2013.
Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin D, Venkatachalam V, Lee H, Cohen AE Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25.
Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782.
Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. Kralj JM, Hochbaum DR, Douglass AD, Cohen AE Science. 2011 Jul 15;333(6040):345-8.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GKS, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE & Cohen AE Nature Methods (2014) doi:10.1038/nmeth.3000
Cohen Lab VIP plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
33780 pJMK001 Proteorhodopsin Optical Proton Sensor Bacterial Expression Kralj et al Science. 2011 Jul 15;333(6040):345-8. pBAD TOPO
34616 pJMK004 Arch D95N Mammalian Expression, Lentiviral Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782. FCK(1.3)
42168 pJMK019 CaViar Mammalian Expression, Lentiviral Cohen Lab VIP plasmids (unpublished) p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
51081 Arch(D95H)-eGFP Archaerhodopsin-3 w/ D95H point mutation fused to eGFP (Other) Mammalian Expression, Lentiviral Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi: 10.1371/journal.pone.0085221. eCollection 2013. Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage FUGW (from Addgene plasmid 22051)
51629 DRH229: FCK-QuasAr1-mO2 QuasAr1-mOrange (Synthetic) Mammalian Expression, Lentiviral Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter for membrane voltage with improved brightness FCK(1.3)
51692 DRH334: FCK-QuasAr2-mO2 QuasAr2-mOrange (Other) Mammalian Expression, Lentiviral Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter of membrane voltage with improved sensitivity FCK(1.3)
51693 DRH313: FCK-CheRiff-eGFP CheRiff-eGFP (Synthetic) Mammalian Expression, Lentiviral Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Channelrhodopsin with improved sensitivity and minimal red light photocurrent FCK(1.3)
51694 DRH296: FCK-Optopatch2 Optopatch2 (Synthetic) Mammalian Expression, Lentiviral Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology FCK(1.3)
51695 DRH335: FCK-Optopatch1 Optopatch1 (Synthetic) Mammalian Expression, Lentiviral Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology FCK(1.3)
51696 DRH322: AAV-CaMKIIa-QuasAr2-mO2 QuasAr2-mOrange (Synthetic) AAV Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 AAV production of QuasAr2 fluorescent reporter pAAV
51697 DRH337: AAV-hsyn-CheRiff-eGFP CheRiff-eGFP (Synthetic) AAV Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 AAV production of CheRiff channelrhodopsin actuator pAAV
58487 VV001: WT Arch-3 in pET28b Archaerhodopsin-3 (with C-terminal His tag) (Other) Bacterial Expression Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli pET28b
58488 VV020: WT Arch-3 - eGFP in pET28b Archaerhodopsin-3 (Other) Bacterial Expression Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli pET28b
58490 VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP Archaerhodopsin-3 w/ D95H point mutation fused to eGFP (Other) Mammalian Expression, Lentiviral Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27. Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage FUGW (from Addgene plasmid 22051)
58500 VV063: 1xCox8 - superecliptic pHluorin in fck 1xCox8 - Superecliptic pHluorin (Synthetic) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells FCK(1.3)GW, from Addgene 22217
58502 VV064: 1xCox8 - ratiometric pHluorin in fck 1xCox8 - Ratiometric pHluorin (Synthetic) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells FCK(1.3)GW, from Addgene 22217
58509 VV073: 1xCox8 - GCaMP5G in fubi 1xCox8 - GCaMP5G (Rattus norvegicus) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the calcium sensor GCaMP5G in the mitochondria FCK(1.3)GW, from Addgene 22217
58510 VV201: ChR2(C128S)-eGFP in fubi ChR2(C128S)-eGFP (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter FUGW (from Addgene plasmid 22051)
58511 VV208: sdChR(C138S,E154A)-TS-eGFP-ER in fck sdChR(C138S, E154A)-TS-eGFP-ER (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR with the C138S (step function opsin) mutation and the E154A mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217
58512 VV220: CoChR(C108S)-eGFP in fck CoChR(C108S)-eGFP (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217
58513 VV221: sdChR(C138S)-TS-eGFP-ER in fck sdChR(C138S)-TS-eGFP-ER (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217
58514 VV229: GCaMP6f in fck GCaMP6f (Rattus norvegicus) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses GCaMP6f under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217
58521 VV246: CoChR(C108S)-GCaMP6f-ER in fck CoChR(C108S)-GCaMP6f-ER (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217
58522 VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck sdChR(C138S)-TS-GCaMP6f-ER (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217
58523 VV228: sdChR(C138S E154A)-TS in fck sdChR(C138S, E154A)-TS (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S E154A) in mammalian cells FCK(1.3)GW, from Addgene 22217
58524 VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck sdChR(C138S, E154A)-TS-GCaMP6f-ER (Other) Mammalian Expression, Lentiviral Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217