Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

Adam Cohen Lab Plasmids


The Adam Cohen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

Learn more about research in the Adam Cohen Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

Title Authors Publication
Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam V, Cohen AE Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020.
Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. Venkatachalam V, Brinks D, Maclaurin D, Hochbaum D, Kralj J, Cohen AE J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27.
Screening fluorescent voltage indicators with spontaneously spiking HEK cells. Park J, Werley CA, Venkatachalam V, Kralj JM, Dib-Hajj SD, Waxman SG, Cohen AE PLoS One. 2013 Dec 31;8(12):e85221. doi: 10.1371/journal.pone.0085221. eCollection 2013.
Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin D, Venkatachalam V, Lee H, Cohen AE Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25.
Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782.
Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. Kralj JM, Hochbaum DR, Douglass AD, Cohen AE Science. 2011 Jul 15;333(6040):345-8.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GKS, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE & Cohen AE Nature Methods (2014) doi:10.1038/nmeth.3000
Cohen Lab VIP plasmids (unpublished)
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pJMK001 Proteorhodopsin Optical Proton Sensor Bacterial Expression Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. Kralj et al Science. 2011 Jul 15;333(6040):345-8. pBAD TOPO Add to Cart
pJMK004 Arch D95N Mammalian Expression, Lentiviral Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782. FCK(1.3) Add to Cart
pJMK019 CaViar Mammalian Expression, Lentiviral Cohen Lab VIP plasmids (unpublished) p156RRL-sinPPT-CMV-GFP-PRE/Nhe I Add to Cart
Arch(D95H)-eGFP Archaerhodopsin-3 w/ D95H point mutation fused to eGFP (Other) Mammalian Expression, Lentiviral Screening fluorescent voltage indicators with spontaneously spiking HEK cells. Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi: 10.1371/journal.pone.0085221. eCollection 2013. Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage FUGW (from Addgene plasmid 22051) Add to Cart
DRH229: FCK-QuasAr1-mO2 QuasAr1-mOrange (Synthetic) Mammalian Expression, Lentiviral All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter for membrane voltage with improved brightness FCK(1.3) Add to Cart
DRH334: FCK-QuasAr2-mO2 QuasAr2-mOrange (Other) Mammalian Expression, Lentiviral All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter of membrane voltage with improved sensitivity FCK(1.3) Add to Cart
DRH313: FCK-CheRiff-eGFP CheRiff-eGFP (Synthetic) Mammalian Expression, Lentiviral All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Channelrhodopsin with improved sensitivity and minimal red light photocurrent FCK(1.3) Add to Cart
DRH296: FCK-Optopatch2 Optopatch2 (Synthetic) Mammalian Expression, Lentiviral All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology FCK(1.3) Add to Cart
DRH335: FCK-Optopatch1 Optopatch1 (Synthetic) Mammalian Expression, Lentiviral All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology FCK(1.3) Add to Cart
DRH322: AAV-CaMKIIa-QuasAr2-mO2 QuasAr2-mOrange (Synthetic) AAV All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 AAV production of QuasAr2 fluorescent reporter pAAV Add to Cart
DRH337: AAV-hsyn-CheRiff-eGFP CheRiff-eGFP (Synthetic) AAV All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum et al Nature Methods (2014) doi:10.1038/nmeth.3000 AAV production of CheRiff channelrhodopsin actuator pAAV Add to Cart
VV001: WT Arch-3 in pET28b Archaerhodopsin-3 (with C-terminal His tag) (Other) Bacterial Expression Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli pET28b Add to Cart
VV020: WT Arch-3 - eGFP in pET28b Archaerhodopsin-3 (Other) Bacterial Expression Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli pET28b Add to Cart
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP Archaerhodopsin-3 w/ D95H point mutation fused to eGFP (Other) Mammalian Expression, Lentiviral Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27. Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage FUGW (from Addgene plasmid 22051) Add to Cart
VV063: 1xCox8 - superecliptic pHluorin in fck 1xCox8 - Superecliptic pHluorin (Synthetic) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
VV064: 1xCox8 - ratiometric pHluorin in fck 1xCox8 - Ratiometric pHluorin (Synthetic) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
VV073: 1xCox8 - GCaMP5G in fubi 1xCox8 - GCaMP5G (Rattus norvegicus) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the calcium sensor GCaMP5G in the mitochondria FCK(1.3)GW, from Addgene 22217 Add to Cart
VV201: ChR2(C128S)-eGFP in fubi ChR2(C128S)-eGFP (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter FUGW (from Addgene plasmid 22051) Add to Cart
VV208: sdChR(C138S,E154A)-TS-eGFP-ER in fck sdChR(C138S, E154A)-TS-eGFP-ER (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR with the C138S (step function opsin) mutation and the E154A mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
VV220: CoChR(C108S)-eGFP in fck CoChR(C108S)-eGFP (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
VV221: sdChR(C138S)-TS-eGFP-ER in fck sdChR(C138S)-TS-eGFP-ER (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
VV229: GCaMP6f in fck GCaMP6f (Rattus norvegicus) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses GCaMP6f under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
VV246: CoChR(C108S)-GCaMP6f-ER in fck CoChR(C108S)-GCaMP6f-ER (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck sdChR(C138S)-TS-GCaMP6f-ER (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
VV228: sdChR(C138S E154A)-TS in fck sdChR(C138S, E154A)-TS (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S E154A) in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck sdChR(C138S, E154A)-TS-GCaMP6f-ER (Other) Mammalian Expression, Lentiviral Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart