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Adam Cohen Lab Plasmids


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Title Authors Publication
Electrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. Kralj JM, Hochbaum DR, Douglass AD, Cohen AE Science. 2011 Jul 15;333(6040):345-8.
Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Kralj JM, Douglass AD, Hochbaum DR, Maclaurin D, Cohen AE Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782.
Cohen Lab VIP plasmids (unpublished)
Screening fluorescent voltage indicators with spontaneously spiking HEK cells. Park J, Werley CA, Venkatachalam V, Kralj JM, Dib-Hajj SD, Waxman SG, Cohen AE PLoS One. 2013 Dec 31;8(12):e85221. doi: 10.1371/journal.pone.0085221. eCollection 2013.
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GKS, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE & Cohen AE Nature Methods (2014) doi:10.1038/nmeth.3000
Imaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Venkatachalam V, Cohen AE Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020.
Bright and fast multicoloured voltage reporters via electrochromic FRET. Zou P, Zhao Y, Douglass AD, Hochbaum DR, Brinks D, Werley CA, Harrison DJ, Campbell RE, Cohen AE Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625.
Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin D, Venkatachalam V, Lee H, Cohen AE Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25.
Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. Venkatachalam V, Brinks D, Maclaurin D, Hochbaum D, Kralj J, Cohen AE J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27.
Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging. Dempsey GT, Chaudhary KW, Atwater N, Nguyen C, Brown BS, McNeish JD, Cohen AE, Kralj JM J Pharmacol Toxicol Methods. 2016 May 13. pii: S1056-8719(16)30044-2. doi: 10.1016/j.vascn.2016.05.003.
A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic Reticulum Calcium Store. Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X, Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK PLoS One. 2015 Oct 9;10(10):e0139273. doi: 10.1371/journal.pone.0139273. eCollection 2015.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
33780pJMK001Proteorhodopsin Optical Proton SensorBacterial ExpressionElectrical spiking in Escherichia coli probed with a fluorescent voltage-indicating protein. Science. 2011 Jul 15;333(6040):345-8. pBAD TOPO Add to Cart
34616pJMK004Arch D95NMammalian Expression, LentiviralOptical recording of action potentials in mammalian neurons using a microbial rhodopsin. Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782. FCK(1.3) Add to Cart
42168pJMK019CaViarMammalian Expression, Lentiviral Cohen Lab VIP plasmids (unpublished) p156RRL-sinPPT-CMV-GFP-PRE/Nhe I Add to Cart
51081Arch(D95H)-eGFPArchaerhodopsin-3 w/ D95H point mutation fused to eGFP (Other)Mammalian Expression, LentiviralScreening fluorescent voltage indicators with spontaneously spiking HEK cells. PLoS One. 2013 Dec 31;8(12):e85221. doi: 10.1371/journal.pone.0085221. eCollection 2013. Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage FUGW (from Addgene plasmid 22051) Add to Cart
51629DRH229: FCK-QuasAr1-mO2QuasAr1-mOrange (Synthetic)Mammalian Expression, LentiviralAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter for membrane voltage with improved brightness FCK(1.3) Add to Cart
51692DRH334: FCK-QuasAr2-mO2QuasAr2-mOrange (Other)Mammalian Expression, LentiviralAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter of membrane voltage with improved sensitivity FCK(1.3) Add to Cart
51693DRH313: FCK-CheRiff-eGFPCheRiff-eGFP (Synthetic)Mammalian Expression, LentiviralAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Channelrhodopsin with improved sensitivity and minimal red light photocurrent FCK(1.3) Add to Cart
51694DRH296: FCK-Optopatch2Optopatch2 (Synthetic)Mammalian Expression, LentiviralAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology FCK(1.3) Add to Cart
51695DRH335: FCK-Optopatch1Optopatch1 (Synthetic)Mammalian Expression, LentiviralAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology FCK(1.3) Add to Cart
51696DRH322: AAV-CaMKIIa-QuasAr2-mO2QuasAr2-mOrange (Synthetic)AAVAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 AAV production of QuasAr2 fluorescent reporter pAAV Add to Cart
51697DRH337: AAV-hsyn-CheRiff-eGFPCheRiff-eGFP (Synthetic)AAVAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 AAV production of CheRiff channelrhodopsin actuator pAAV Add to Cart
58487VV001: WT Arch-3 in pET28bArchaerhodopsin-3 (with C-terminal His tag) (Other)Bacterial ExpressionMechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli pET28b Add to Cart
58488VV020: WT Arch-3 - eGFP in pET28bArchaerhodopsin-3 (Other)Bacterial ExpressionMechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli pET28b Add to Cart
58490VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFPArchaerhodopsin-3 w/ D95H point mutation fused to eGFP (Other)Mammalian Expression, LentiviralFlash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. J Am Chem Soc. 2014 Feb 12;136(6):2529-37. doi: 10.1021/ja411338t. Epub 2014 Jan 27. Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage FUGW (from Addgene plasmid 22051) Add to Cart
58500VV063: 1xCox8 - superecliptic pHluorin in fck1xCox8 - Superecliptic pHluorin (Synthetic)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
58502VV064: 1xCox8 - ratiometric pHluorin in fck1xCox8 - Ratiometric pHluorin (Synthetic)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
58509VV073: 1xCox8 - GCaMP5G in fubi1xCox8 - GCaMP5G (Rattus norvegicus)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses the calcium sensor GCaMP5G in the mitochondria FCK(1.3)GW, from Addgene 22217 Add to Cart
58510VV201: ChR2(C128S)-eGFP in fubiChR2(C128S)-eGFP (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter FUGW (from Addgene plasmid 22051) Add to Cart
58511VV208: sdChR(C138S,E154A)-TS-eGFP-ER in fcksdChR(C138S, E154A)-TS-eGFP-ER (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR with the C138S (step function opsin) mutation and the E154A mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
58512VV220: CoChR(C108S)-eGFP in fckCoChR(C108S)-eGFP (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
58513VV221: sdChR(C138S)-TS-eGFP-ER in fcksdChR(C138S)-TS-eGFP-ER (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
58514VV229: GCaMP6f in fckGCaMP6f (Rattus norvegicus)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses GCaMP6f under the a-CamKII promoter FCK(1.3)GW, from Addgene 22217 Add to Cart
58521VV246: CoChR(C108S)-GCaMP6f-ER in fckCoChR(C108S)-GCaMP6f-ER (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
58522VV247: sdChR(C138S)-TS-GCaMP6f-ER in fcksdChR(C138S)-TS-GCaMP6f-ER (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
58523VV228: sdChR(C138S E154A)-TS in fcksdChR(C138S, E154A)-TS (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S E154A) in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
58524VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fcksdChR(C138S, E154A)-TS-GCaMP6f-ER (Other)Mammalian Expression, LentiviralImaging GFP-Based Reporters in Neurons with Multiwavelength Optogenetic Control. Biophys J. 2014 Oct 7;107(7):1554-63. doi: 10.1016/j.bpj.2014.08.020. Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells FCK(1.3)GW, from Addgene 22217 Add to Cart
59172FCK-QuasAr2-CitrineQuasAr2-Citrine eFRET (Synthetic)Mammalian Expression, LentiviralBright and fast multicoloured voltage reporters via electrochromic FRET. Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625. Fluorescent membrane voltage sensor FCK(1.3) Add to Cart
59173FCK-QuasAr2-mOrange2 eFRETQuasAr2-mOrange2 eFRET (Synthetic)Mammalian Expression, LentiviralBright and fast multicoloured voltage reporters via electrochromic FRET. Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625. Fluorescent membrane voltage sensor FCK(1.3) Add to Cart
59174FCK-QuasAr2-mRuby2 eFRETQuasAr2-mRuby2 eFRET (Synthetic)Mammalian Expression, LentiviralBright and fast multicoloured voltage reporters via electrochromic FRET. Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625. Fluorescent membrane voltage sensor FCK(1.3) Add to Cart
62984pMOS001: CMV-Optopatch2_FCKOptopatch2 (Synthetic)Mammalian Expression, LentiviralAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter FCK(1.3) Add to Cart
62985pMOS009: pLX304-QuasAr2QuasAr2-mOrangeMammalian Expression, Lentiviral ; Gateway Destination vectorAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Lentivirus production of QuasAr2 fluorescent reporter pLX304 Add to Cart
62986pMOS010: pLX304-CheRiff-GFPCheRiff-eGFP (Synthetic)Mammalian Expression, Lentiviral ; Gateway Destination vectorAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Lentivirus production of CheRiff channelrhodopsin actuator pLX304 Add to Cart
64134pBAD_His B_QuasAr2QuasAr2 (Synthetic)Bacterial ExpressionAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter of membrane voltage with improved sensitivity pBAD Add to Cart
64135pBAD_His B_QuasAr1QuasAr1 (Synthetic)Bacterial ExpressionAll-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins Nature Methods (2014) doi:10.1038/nmeth.3000 Fluorescent reporter for membrane voltage with improved brightness pBAD Add to Cart
65227pMOS003-lenti-CMV-GCaMPerGCaMPer (Synthetic)Mammalian Expression, LentiviralA Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic Reticulum Calcium Store. PLoS One. 2015 Oct 9;10(10):e0139273. doi: 10.1371/journal.pone.0139273. eCollection 2015. ER calcium sensor for lentivirus production FCK Add to Cart
72303pJMK074: CMV QuasAr2-TS-GCaMP6fCaViar (Synthetic)Mammalian Expression, LentiviralCardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging. J Pharmacol Toxicol Methods. 2016 May 13. pii: S1056-8719(16)30044-2. doi: 10.1016/j.vascn.2016.05.003. Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator p156RRL-sinPPT-CMV-GFP-PRE/Nhe I Add to Cart
72304pJMK077: CaMKIIa QuasAr2-TS-GCaMP6fCaViar (Synthetic)Mammalian Expression, LentiviralCardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging. J Pharmacol Toxicol Methods. 2016 May 13. pii: S1056-8719(16)30044-2. doi: 10.1016/j.vascn.2016.05.003. Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator FCK Add to Cart