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Alex Schier Lab Plasmids


The Alex Schier Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
47322pCS2-Cas9Cas9 (Other)Mammalian Expression, CRISPR ; xenopus and zebrafish expressionEfficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. For in vivo expression of Cas9 or generation of Cas9 mRNA pCS2 Add to Cart
47327pET-28b-Cas9-HisCas9 (Other)Bacterial Expression, CRISPREfficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. For in vitro expression and purification of Cas9 protein pET-28b Add to Cart