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Alexandra Newton Lab Plasmids


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Title Authors Publication
A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. Violin JD, Zhang J, Tsien RY, Newton AC J Cell Biol. 2003 Jun 9. 161(5):899-909.
Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. Gallegos LL, Kunkel MT, Newton AC J Biol Chem. 2006 Oct 13. 281(41):30947-56.
Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. Kunkel MT, Ni Q, Tsien RY, Zhang J, Newton AC J Biol Chem. 2005 Feb 18. 280(7):5581-7.
Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. Kunkel MT, Toker A, Tsien RY, Newton AC J Biol Chem. 2007 Mar 2. 282(9):6733-42.
PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Brognard J, Sierecki E, Gao T, Newton AC Mol Cell. 2007 Mar 23. 25(6):917-31.
PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Gao T, Furnari F, Newton AC Mol Cell. 2005 Apr 1. 18(1):13-24.
Protein kinase C {delta}-specific activity reporter reveals agonist-evoked nuclear activity controlled by Src family of kinases. Kajimoto T, Sawamura S, Tohyama Y, Mori Y, Newton AC J Biol Chem. 2010 Dec 31. 285(53):41896-910.
The protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. Kunkel MT, Garcia EL, Kajimoto T, Hall RA, Newton AC J Biol Chem. 2009 Sep 4. 284(36):24653-61.
Mislocalization of the E3 ligase, beta-transducin repeat-containing protein 1 (beta-TrCP1), in glioblastoma uncouples negative feedback between the pleckstrin homology domain leucine-rich repeat protein phosphatase 1 (PHLPP1) and Akt. Warfel NA, Niederst M, Stevens MW, Brennan PM, Frame MC, Newton AC J Biol Chem. 2011 Jun 3;286(22):19777-88. Epub 2011 Mar 28.
Spatiotemporally distinct protein kinase D activation in adult cardiomyocytes in response to phenylephrine and endothelin. Bossuyt J, Chang CW, Helmstadter K, Kunkel MT, Newton AC, Campbell KS, Martin JL, Bossuyt S, Robia SL, Bers DM J Biol Chem. 2011 Sep 23;286(38):33390-400. doi: 10.1074/jbc.M111.246447. Epub 2011 Jul 27.
Calcium transduces plasma membrane receptor signals to produce diacylglycerol at Golgi membranes. Kunkel MT, Newton AC J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 10.1074/jbc.C110.123133. Epub 2010 Jun 2.
Pleckstrin homology domain leucine-rich repeat protein phosphatases set the amplitude of receptor tyrosine kinase output. Reyes G, Niederst M, Cohen-Katsenelson K, Stender JD, Kunkel MT, Chen M, Brognard J, Sierecki E, Gao T, Nowak DG, Trotman LC, Glass CK, Newton AC Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):E3957-65. doi: 10.1073/pnas.1404221111. Epub 2014 Sep 8.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
14860CKARC kinase activity reporterMammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Add to Cart
14861CKAR-T/AC kinase activity reporterMammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Add to Cart
14862MyrPalm-CKARC kinase activity reporterMammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Add to Cart
14863MyrPalm-CKAR-T/AC kinase activity reporterMammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Add to Cart
14864CYPHRcyan/yellow PH domain reporterMammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Plcd1 AW212592, C79986 Add to Cart
14865DAGRDAG reporter (Rattus norvegicus)Mammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Prkcb Pkcb, Prkcb1 Add to Cart
14866YFP-PKCbetaII-YFPYFP-PKCbetaII-YFP (Rattus norvegicus)Mammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Add to Cart
14867MyrPalm-CFPCFPMammalian ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pcDNA3 Add to Cart
14868pRSET B-CKARC kinase activity reporterBacterial ExpressionA genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003 Jun 9. 161(5):899-909. pRSET B Add to Cart
14869NucCKARC kinase activity reporterMammalian ExpressionTargeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006 Oct 13. 281(41):30947-56. pcDNA3 Add to Cart
14870CytoCKARC kinase activity reporterMammalian ExpressionTargeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006 Oct 13. 281(41):30947-56. pcDNA3 Add to Cart
14871GolgiCKARC kinase activity reporterMammalian ExpressionTargeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006 Oct 13. 281(41):30947-56. pcDNA3 Add to Cart
14872MitoCKARC kinase activity reporterMammalian ExpressionTargeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006 Oct 13. 281(41):30947-56. pcDNA3 Add to Cart
14873GolgiCFPCFPMammalian ExpressionTargeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006 Oct 13. 281(41):30947-56. pcDNA3 Add to Cart
14874YFP-DBDDAG binding domain (Rattus norvegicus)Mammalian ExpressionTargeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006 Oct 13. 281(41):30947-56. pcDNA3 Prkcb Pkcb, Prkcb1 Add to Cart
14875BKARB kinase activity reporterMammalian ExpressionSpatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem. 2005 Feb 18. 280(7):5581-7. pcDNA3 Add to Cart
14876BKAR-T/AB kinase activity reporterMammalian ExpressionSpatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem. 2005 Feb 18. 280(7):5581-7. pcDNA3 Add to Cart
14877pRSET B-BKARB kinase activity reporterBacterial ExpressionSpatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem. 2005 Feb 18. 280(7):5581-7. pRSET B Add to Cart
14878MyrPalm-BKARB kinase activity reporterMammalian ExpressionSpatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem. 2005 Feb 18. 280(7):5581-7. pcDNA3 Add to Cart
14879DKARD kinase activity reporterMammalian ExpressionCalcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. J Biol Chem. 2007 Mar 2. 282(9):6733-42. pcDNA3 Add to Cart
14880DKAR-T/AD kinase activity reporterMammalian ExpressionCalcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. J Biol Chem. 2007 Mar 2. 282(9):6733-42. pcDNA3 Add to Cart
14881pRSET B-DKARD kinase activity reporterBacterial ExpressionCalcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter. J Biol Chem. 2007 Mar 2. 282(9):6733-42. pRSET B Add to Cart
22403pcDNA3-HA-PHLPP2PHLPP2 (Homo sapiens)Mammalian ExpressionPHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol Cell. 2007 Mar 23. 25(6):917-31. pcDNA3 PHLPP2 PHLPPL, PPM3B Add to Cart
22404pcDNA3-HA-PHLPP1 N-terminal truncationPHLPP1 (Homo sapiens)Mammalian ExpressionPHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol Cell. 2005 Apr 1. 18(1):13-24. Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells pcDNA3 PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP Add to Cart
22930pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPHPHLPP1-deltaPH (Homo sapiens)Mammalian ExpressionPHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol Cell. 2005 Apr 1. 18(1):13-24. Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain pcDNA3 PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP Add to Cart
22931pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaCPHLPP1-deltaC (Homo sapiens)Mammalian ExpressionPHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol Cell. 2005 Apr 1. 18(1):13-24. Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C) pcDNA3 PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP Add to Cart
31526pcDNA3-deltaCKARDelta-selective C kinase activity reporterMammalian ExpressionProtein kinase C {delta}-specific activity reporter reveals agonist-evoked nuclear activity controlled by Src family of kinases. J Biol Chem. 2010 Dec 31. 285(53):41896-910. pcDNA3 Add to Cart
31527pcDNA3-YFP-PKD1YFP-HA-Protein Kinase D (Homo sapiens)Mammalian ExpressionThe protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. J Biol Chem. 2009 Sep 4. 284(36):24653-61. pcDNA3 PRKD1 CHDED, PKC-MU, PKCM, PKD, PRKCM Add to Cart
31821pcDNA3-YFP-PKD2YFP-Protein Kinase D2 (Homo sapiens)Mammalian ExpressionThe protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. J Biol Chem. 2009 Sep 4. 284(36):24653-61. pcDNA3
  • Tag / Fusion Protein
    • YFP (N terminal on insert)
  • Add to Cart
    37100pcDNA3 HA-PHLPP1 full lengthPHLPP1 (Homo sapiens)Mammalian ExpressionMislocalization of the E3 ligase, beta-transducin repeat-containing protein 1 (beta-TrCP1), in glioblastoma uncouples negative feedback between the pleckstrin homology domain leucine-rich repeat protein phosphatase 1 (PHLPP1) and Akt. J Biol Chem. 2011 Jun 3;286(22):19777-88. Epub 2011 Mar 28. Expresses HA tagged, full-length human PHLPP1 in mammalian cells pcDNA3 PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP Add to Cart
    45499pcDNA3-DKAR-NucDKAR-Nuc (Synthetic)Mammalian ExpressionSpatiotemporally distinct protein kinase D activation in adult cardiomyocytes in response to phenylephrine and endothelin. J Biol Chem. 2011 Sep 23;286(38):33390-400. doi: 10.1074/jbc.M111.246447. Epub 2011 Jul 27. pcDNA3 Add to Cart
    49790Golgi-DKARD kinase activity reporter (Synthetic)Mammalian ExpressionCalcium transduces plasma membrane receptor signals to produce diacylglycerol at Golgi membranes. J Biol Chem. 2010 Jul 23;285(30):22748-52. doi: 10.1074/jbc.C110.123133. Epub 2010 Jun 2. FRET-based, Golgi-targeted kinase activity reporter for PKD pcDNA3 Add to Cart
    84824pBabe-HA-PHLPP1-delta-PHphlpp1 (Homo sapiens)RetroviralPleckstrin homology domain leucine-rich repeat protein phosphatases set the amplitude of receptor tyrosine kinase output. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):E3957-65. doi: 10.1073/pnas.1404221111. Epub 2014 Sep 8. Mammalian expression of a HA tagged, human PHLPP1 containing a deletion of the PH domain (delta PH) residues Q551 to R638 were deleted pBabe puro PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP Add to Cart
    84825pBabe-HA-PHLPP1-delta-PDZphlpp1 (Homo sapiens)RetroviralPleckstrin homology domain leucine-rich repeat protein phosphatases set the amplitude of receptor tyrosine kinase output. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):E3957-65. doi: 10.1073/pnas.1404221111. Epub 2014 Sep 8. Mammalian expression of a HA tagged, human PHLPP1 containing a deletion of the PDZ binding motif pBabe puro PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP Add to Cart