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Caixia Gao Lab Plasmids


The Caixia Gao Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Targeted genome modification of crop plants using a CRISPR-Cas system. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System. Liang Z, Zhang K, Chen K, Gao C J Genet Genomics. 2014 Feb 20;41(2):63-8. doi: 10.1016/j.jgg.2013.12.001. Epub 2013 Dec 14.
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Liang Z, Chen K, Li T, Zhang Y, Wang Y, Zhao Q, Liu J, Zhang H, Liu C, Ran Y, Gao C Nat Commun. 2017 Jan 18;8:14261. doi: 10.1038/ncomms14261.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
53061pZmU3-gRNAzmU3 promoter - gRNA (Other)CRISPR ; Plant expressionTargeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System. J Genet Genomics. 2014 Feb 20;41(2):63-8. doi: 10.1016/j.jgg.2013.12.001. Epub 2013 Dec 14. Transcription of guide RNA (gRNA) in maize cells pEasy-blunt Add to Cart
53062pU6-gRNAwheat U6 promoter - gRNA (Other)CRISPR ; Plant expressionTargeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. Transcription of guide RNA (gRNA) in wheat cells pUC-T Add to Cart
53063pU3-gRNAOsU3 promoter + gRNA scaffold (Other)CRISPR ; Plant expressionTargeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. Transcription of guide RNA (gRNA) in rice cells pUC-T Add to Cart
53064pJIT163-2NLSCas9Cas9 (Other)Plant expressionTargeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650. Expression of rice codon-optimized Cas9 in plant cells pJIT163 Add to Cart
98158pET28a-Cas9-HisNLS-Cas9-NLS (Other)Bacterial ExpressionEfficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Nat Commun. 2017 Jan 18;8:14261. doi: 10.1038/ncomms14261. Purification of Cas9 protein pET28a (+) Add to Cart
98161pdCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSPlant ExpressionPrecise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP Add to Cart
98162pH-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSPlant Expression ; RicePrecise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411 Add to Cart
98164pnCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSPlant ExpressionPrecise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP Add to Cart