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Carl Novina Lab Plasmids


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Title Authors Publication
Recapitulation of short RNA-directed translational gene silencing in vitro. Wang B, Love TM, Call ME, Doench JG, Novina CD Mol Cell. 2006 May 19. 22(4):553-60.
Distinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Wang B, Li S, Qi HH, Chowdhury D, Shi Y, Novina CD Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66.
Intronic miR-211 assumes the tumor suppressive function of its host gene in melanoma. Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, Granter SR, Turley SJ, Fisher DE, Novina CD Mol Cell. 2010 Dec 10. 40(5):841-9.
Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, Novina CD, Orth AP Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins. Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD RNA. 2012 Nov;18(11):2041-55. doi: 10.1261/rna.035675.112. Epub 2012 Sep 27.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12563PCD FL0XMammalian Expression, Luciferase ; Reporter for translation repressionRecapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006 May 19. 22(4):553-60. pcDNA3.1 Add to Cart
12564PCD FL2XTwo CXCR4 imperfect binding sites (Homo sapiens)Mammalian Expression, Luciferase ; Reporter for translation repressionRecapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006 May 19. 22(4):553-60. pcDNA3.1 CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS Add to Cart
12565PCD FL4XFour CXCR4 imperfect binding sites (Homo sapiens)Mammalian Expression, Luciferase ; Reporter for translation repressionRecapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006 May 19. 22(4):553-60. pcDNA3.1 CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS Add to Cart
12566PCD FL6XFour CXCR4 imperfect binding sites (Homo sapiens)Mammalian Expression, Luciferase ; Reporter for translation repressionRecapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006 May 19. 22(4):553-60. pcDNA3.1 CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS Add to Cart
12567PCD FL1POne perfect CXCR4 binding site (Homo sapiens)Mammalian Expression, Luciferase ; Reporter for translation repressionRecapitulation of short RNA-directed translational gene silencing in vitro. Mol Cell. 2006 May 19. 22(4):553-60. pcDNA3.1 CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS Add to Cart
24316pGEX-GST-AGO1human AGO1 (Homo sapiens)Bacterial ExpressionDistinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. pGEX-4T1 AGO1 EIF2C, EIF2C1, GERP95, Q99, hAgo1 Add to Cart
24317pGEX-GST-AGO2human AGO2 (Homo sapiens)Bacterial ExpressionDistinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. pGEX-4T-1 AGO2 EIF2C2, Q10 Add to Cart
24318pGEX-GST-AGO3AGO3 (Homo sapiens)Bacterial ExpressionDistinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. pGEX-4T-1 AGO3 RP4-789D17.2, EIF2C3 Add to Cart
24319pGEX-GST-AGO4AGO4 (Homo sapiens)Bacterial ExpressionDistinct passenger strand and mRNA cleavage activities of human Argonaute proteins. Nat Struct Mol Biol. 2009 Dec . 16(12):1259-66. pGEX-4T-1 AGO4 EIF2C4 Add to Cart
29780PLKO-mcherry-luc-puro-shSCRshSCRMammalian Expression, Lentiviral, RNAiIntronic miR-211 assumes the tumor suppressive function of its host gene in melanoma. Mol Cell. 2010 Dec 10. 40(5):841-9. PLKO (modified) Add to Cart
29782PLKO-mcherry-luc-puro-TRPM1 cDNATRPM1 (Homo sapiens)Mammalian Expression, LentiviralIntronic miR-211 assumes the tumor suppressive function of its host gene in melanoma. Mol Cell. 2010 Dec 10. 40(5):841-9. PLKO (modified) TRPM1 CSNB1C, LTRPC1, MLSN1 Add to Cart
29783PLKO-mcherry-luc-puro-Renilla cDNARenillaMammalian Expression, LentiviralIntronic miR-211 assumes the tumor suppressive function of its host gene in melanoma. Mol Cell. 2010 Dec 10. 40(5):841-9. PLKO modified Add to Cart
29784PLKO-mcherry-luc-puro-miR-211miR-211Mammalian Expression, Lentiviral, RNAiIntronic miR-211 assumes the tumor suppressive function of its host gene in melanoma. Mol Cell. 2010 Dec 10. 40(5):841-9. PLKO (modified) Add to Cart
59904pDEST26 Ago2Argonaute 2 (Homo sapiens)Mammalian ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells pDEST 26 AGO2 EIF2C2, Q10 Add to Cart
59905pDEST26 Ago2 S387AArgonaute 2 (Homo sapiens)Mammalian ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells pDEST 26 AGO2 EIF2C2, Q10 Add to Cart
59906pDEST26 Ago2 S387EArgonaute 2 (Homo sapiens)Mammalian ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells pDEST 26 AGO2 EIF2C2, Q10 Add to Cart
59907pDEST26 Ago2 4AArgonaute 2 (Homo sapiens)Mammalian ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells pDEST 26 AGO2 EIF2C2, Q10 Add to Cart
60651pDEST26 Ago2 5AArgonaute 2 (Homo sapiens)Mammalian ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells pDEST 26 AGO2 EIF2C2, Q10 Add to Cart
60652pcDNA 3.1 (+) FLAG-Ago2 S387AArgonaute 2 (Homo sapiens)Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells 5428 AGO2 EIF2C2, Q10 Add to Cart
60653pcDNA 3.1 (+) FLAG-Ago2 S387EArgonaute 2 (Homo sapiens)Mammalian ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells pcDNA3.1(+) AGO2 EIF2C2, Q10 Add to Cart
60654pHAGE GFP-Ago2 S387AArgonaute 2 (Homo sapiens)LentiviralAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. GFP-Ago2 S387A mutant lentiviral vector pHAGE AGO2 EIF2C2, Q10 Add to Cart
60655pHAGE GFP-Ago2 S387EArgonaute 2 (Homo sapiens)LentiviralAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. GFP-Ago2 S387E mutant lentiviral vector pHAGE AGO2 EIF2C2, Q10 Add to Cart
60656pGEX 4T-1 GST-Ago2 S387AArgonaute 2 (Homo sapiens)Bacterial ExpressionAkt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets. Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. Expresses GST-Ago2 S387A mutant in mammalian cells pGEX 4T-1 AGO2 EIF2C2, Q10 Add to Cart
74231pHAGE-eGFP-Ago2Ago2 (Homo sapiens)LentiviralAlternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins. RNA. 2012 Nov;18(11):2041-55. doi: 10.1261/rna.035675.112. Epub 2012 Sep 27. GFP-Ago2 lentiviral vector pHAGE AGO2 EIF2C2, Q10 Add to Cart